fluorogenic hdac 1  (BPS Bioscience)

Journal: Medicine

Article Title: Design, synthesis, and biological evaluation of histone deacetylase inhibitor with novel salicylamide zinc binding group

doi: 10.1097/MD.0000000000029049

Figure Lengend Snippet: In vitro inhibitory activity of compound 5 against histone deacetylase isotypes.

Article Snippet: HDAC fluorogenic assay kits (HDAC1, #50061; HDAC2, #50062; HDAC3, #50073; HDAC4, #50064; HDAC6, #50076; HDAC11, #50687) were bought from BPS Bioscience (San Diego, CA).

Techniques: In Vitro, Activity Assay, Histone Deacetylase Assay

Journal: Medicine

Article Title: Design, synthesis, and biological evaluation of histone deacetylase inhibitor with novel salicylamide zinc binding group

doi: 10.1097/MD.0000000000029049

Figure Lengend Snippet: Effect of compound 5 on the acetylation status of Histone H3 and α-tubulin. MDA-MB-231 cells were incubated with the indicated concentrations of compound 5 for 24 hours and the expression level of Histone H3, Ac-Histone H3, α-tubulin, Ac-α-tubulin, HDAC1, HDAC6, and GAPDH were analyzed using Western blotting. DMSO (D) was used as a negative control. GAPDH = glyceraldehyde 3-phosphate dehydrogenase, HDAC = Histone deacetylase.

Article Snippet: HDAC fluorogenic assay kits (HDAC1, #50061; HDAC2, #50062; HDAC3, #50073; HDAC4, #50064; HDAC6, #50076; HDAC11, #50687) were bought from BPS Bioscience (San Diego, CA).

Techniques: Incubation, Expressing, Western Blot, Negative Control, Histone Deacetylase Assay

Journal: Medicine

Article Title: Design, synthesis, and biological evaluation of histone deacetylase inhibitor with novel salicylamide zinc binding group

doi: 10.1097/MD.0000000000029049

Figure Lengend Snippet: Molecular docking pose of compound 5 bound in the substrate-binding pocket of HDAC2 (PDB code: 4LXZ). The docking pose of compound 5 (lime) and the crystal structure of SAHA (pink) complex were overlapped in the substrate-binding pocket of HDAC2. The oxygen, nitrogen, and hydrogen atoms of compound 5 and SAHA are shown in red, blue, and white, respectively. The side chains of the binding site are colored according to the atom types (carbon, light blue; oxygen, red; nitrogen, blue) and labeled with their residue name. The hydrogen bonds are shown as dashed red lines. The docking poses are visualized using PyMOL1.3.

Article Snippet: HDAC fluorogenic assay kits (HDAC1, #50061; HDAC2, #50062; HDAC3, #50073; HDAC4, #50064; HDAC6, #50076; HDAC11, #50687) were bought from BPS Bioscience (San Diego, CA).

Techniques: Binding Assay, Labeling